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glycoprotein; Microbial transformation of heavy elements in the aquatic environmentKozai, Naofumi; Sakamoto, Fuminori; Tanaka, Kazuya; Onuki, Toshihiko; Sato, Takahiro*; Kamiya, Tomihiro*; Grambow, B.
Chemosphere, 196, p.135 - 144, 2018/04
Times Cited Count：5 Percentile：17.39(Environmental Sciences)Transformation of heavy elements by microbes such as bacteria and fungi has been an intense research subject; however, little is known about that of protozoa. This study investigated interaction of a representative protozoa, , with heavy elements (Eu(III), Pb(II), U(VI)). Non-destructive elemental analysis by micro-PIXE hardly detected those elements on living cells after sorption experiments but clearly detected on the cells that were killed with a fixative beforehand. Chromatographic analysis of aquatic species of those heavy elements after the sorption experiments revealed a fraction of those elements bound to a glycoprotein dissolved from the cell surface of living cells to form soluble pseudocolloid. These findings suggest that complexation of heavy elements with the dissolved surface glycoprotein reduced the sorption of those heavy elements on living cells.
Kozai, Naofumi; Sakamoto, Fuminori; Onuki, Toshihiko; Sato, Takahiro; Koka, Masashi; Kamiya, Tomihiro; Esaka, Fumitaka
no journal, ,
To reveal the role of protozoa in environmental migration of radionuclides, this study investigated behavior of heavy elements (U, Eu) during culture of Paramecium sp. with yeast on which heavy elements were sorbed. Paramecium was chosen as model protozoa. After the exposure of yeast cells to a dilute heavy element aqueous solution, phosphates of these elements formed on yeast cells. Paramecium cells were cultured with those yeast cells. Only very small fractions of the presorbed heavy elements were dissolved from yeast cells during the Paramecium culture. As culture time advances, membranous precipitates formed which contained undigested and digested yeast cells and dense membranous organic substance filling gaps between those cells. In the precipitates many phosphate particles which size and morphology are similar to those on the yeast cells were observed. These results suggest that the immobilization effect of yeast cells for heavy elements was preserved in the prey-predator system.
